Identification Of katG Gene from Mycobacterium Growth Indicator Tube (MGIT) Culture

Abstract

Tuberculosis (TB) is a respiratory infection of the respiratory tract caused by Mycobacterium tuberculosis. There are currently many strains of M. tuberculosis resistant two or more anti-tuberculosis drugs (OAT) known as the Multiple Drugs Resistant M. tuberculosis (MDR-MTB ). One of the marker genes for MDR-MTB is the katG gene. KatG gene mutations cause loss of the activity of the Mycobacterium tuberculosiscatalase - peroxidase enzyme so that these bacteria become resistant to isoniazid (INH) OAT.The purpose of this research is to know the existence of katG genes in isolate Mycobacterium tuberculosis from culture MGIT. Sample used is MGIT isolate with amount of 25 samples then amplification using PCR methode and electrophoresis. The results showed that there were 4 DNA samples suspected by katG genes measuring ± 317 bp from MGIT cultured samples. Based on the results of this study it can be concluded that the katG gene was successfully amplified by PCR method with primary forward and reverse primer visualized by electrophoresis.